These instructions allow to quantitatively assess stress in corals by measuring oxygen production (photosynthesis) of its algal symbionts.

Using the Zymo Quick-DNA/RNA Miniprep Plus Kit on Stylophora pistillata shallow and mesophotic samples from the pH experiment.

These instructions allow to quantitatively assess coral physiological parameters, i.e. tissue biomass (protein concentration), algal density and chlorophyll concentration.

These instructions cover two ways to get algal cell counts using Fiji, one manual and one automated. A fluorescence microscope (Nikon Eclipse Ti, Japan) was used to image symbiotic algae (isolated from coral tissue) both in brightfield and in fluorescent light using 440 nm emission, to identify chlorophyll and to ensure counting of symbiont cells only.